Extracellular Vesicle Biogenesis and Functions in Gram-Positive Bacteria

Cell Envelope Structure and Functions in Gram-negative Bacteria

Vti1a functions in dense-core vesicle biogenesis

The SNARE-protein Vti1a is proposed to drive fusion of intracellular organelles, but recent data also implicated Vti1a in exocytosis. Here we show that vti1a is absent from mature secretory vesicles in adrenal CCs, but localizes to a compartment near the Trans-Golgi-Network, partially overlapping with Syntaxin6. Exocytosis is impaired in vti1a null cells, partly due to fewer Ca-channels at the ...

Synthesis and Characterization of Novel Antibacterial PDDA/Honey Nanofiber Against Gram-Positive and Gram-Negative Bacteria

Nanomaterials are increasingly used to the targeting of gram-positive and gram-negative bacteria as an alternative to antibiotics. Bacterial infections are a major cause of chronic infections and mortality. People requirement for new materials for pathogenic bacteria treatment. It seems that nanomaterial-based strategies can be resolving this problem. In this research, improved antibacterial na...

Isolation and identification of membrane vesicle-associated proteins in Gram-positive bacteria and mycobacteria

Many intracellular bacterial pathogens naturally release membrane vesicles (MVs) under a variety of growth environments. For pathogenic bacteria there are strong evidences that released MVs are a delivery mechanism for the release of immunologically active molecules that contribute to virulence. Identification of membrane vesicle-associated proteins that can act as immunological modulators is c...

Identification and analysis of recombineering functions from Gram-negative and Gram-positive bacteria and their phages.

We report the identification and functional analysis of nine genes from Gram-positive and Gram-negative bacteria and their phages that are similar to lambda (lambda) bet or Escherichia coli recT. Beta and RecT are single-strand DNA annealing proteins, referred to here as recombinases. Each of the nine other genes when expressed in E. coli carries out oligonucleotide-mediated recombination. To o...